Interestingly, knocking down striatal D2R increases the emergence of compulsive-like eating in rats with access to palatable food , and this compulsive behavior continues in the presence of an aversive conditioned stimulus pointing out also the part of CeA . feeding behavior. knockdown in neurons expressing single-minded 1, a transcription element abundantly indicated in the PVN in mice, reduced anxiety-like behavior . However, chemogenetically activation of hindbrain GLP1 neurons shows no effect on anxiety-like behaviors, neither plasma corticosterone levels, showing the importance of hypothalamic GLP1R signaling for behavioral stress reactions in mice . However, the CeA, a mind region essential for the initiation of the stress response , appears critical for generating the anxiogenic effects of GLP-1 since the administration of the peptide in CeA does not improve plasma corticosterone levels but decreases the time spent in the open arms of the EMP . Another neural substrate for GLP-1 control of anxiety-like behavior is the SuM. Selective activation of SuM, with Ex lover-4, decreases the time spent in the center of the open field market in both male and female rats . Normally, initiation of fear and sustained panic reactions requires the recruitment of the BNST , knocking down the translation of GLP1-R mRNA in the anterolateral BNST in rats, decreases anxiety-like behavior in the open field test, including a loss of light-enhanced acoustic startle . Moreover, the central administration of GLP-1 induces anxiety-like behavior in rats . Also, central GLP-1 generates a proconflict effect in the punished drinking test while leaving activity and nociception actions unaffected, assisting an anxiogenic effect . Besides, IU1-47 acute intraperitoneal, central or intra-dorsal raphe GLP-1 of or Ex lover-4 administration raises anxiety-like behavior using three different measuring checks in rats . IU1-47 In contrast, chronic daily central treatment with the Ex lover-4 does not affect anxiety-like behavior but instead reduces depression-like behavior in the push swim test (FST) . Contrarily, in humans, intravenously given GLP-1 does not appear to possess anxiogenic or panicogenic properties, actually in individuals with panic disorder . Significantly, GLP-1 not just modulates the acute stress response, but can regulate HPA responsiveness to chronic stress. Exposure to chronic stress reduces PPG mRNA manifestation inside a glucocorticoid-dependent manner, indicating that glucocorticoids create long-term PPG downregulation and long-lasting reduction in PPG action , pointing out a role of GLP-1 in stress adaptation. Moreover, GLP-1 is involved in chronic stress-induced facilitation of corticosterone reactions to a novel stressor, since the part of GLP-1 appears to be manifest following different stress exposure . GLP-1 activity may amplify the effects of chronic stress on the organism. The i.c.v chronic administration decreases body weight in animals exposed to chronic stress, even though the GLP-1 administration itself does not precipitate chronic stress-like effects or long term INCENP HPA hyperactivity . In contrast, sub-chronic Ex lover4 administration (subcutaneous bolus) generates several effects that resemble chronic stress. Overactivates, the HPA axis disrupts circadian glucocorticoid secretion, induces hypertrophy of the adrenal gland, decreases its sensitivity, impairs pituitary-adrenal stress reactions induces reductions in both food intake and body weight . Moreover, all those effects were abolished by adrenalectomy . The rules of the HPA axis by GLP-1 or Ex lover4 is definitely independent of the metabolic state in rats . In fasting, during which basal IU1-47 corticosterone levels are high, these peptides induce designated elevations of corticosterone levels, acting in conditions of metabolic stress, and individually of glycemic changes insulinotropic properties . Difficulties in the homeostasis induced by interoceptive stress activate central GLP-1 pathways . The intraperitoneal treatment with the toxin lithium chloride (LiCl) activates c-Fos manifestation of GLP-1 neurons, including those with axonal projections to PVN in rats . The administration of LiCl induces a pool of specific symptoms and behaviors in rats that have been used as indications of visceral illness . Several of these reactions also were caused by GLP-1, such as reduction of food intake [173,174] or conditioned taste aversion (CTA) [173,175]. The GLP-1R antagonist blocks the effect of LiCl to reduce food intake, induces pica, and generates a CTA in rats . Like the rat, LiCl activates PPG-neurons, induces anorexia, and CTA formation in wild-type mice, but LiCl does not evoke aversive effects.